The nanotechnology of create new design of drugs and biophysical methods observation on formation microbial biofilms.

Author: Tamaz Mdzinarashvili
Co-authors: Eka Sekiladze, Mariam Khvedelidze, Elene Lomadze, Irine Papukashvili, Nino Turkadze
Keywords: nanotechnology, nanoparticles, liposoms, bacteria biofilmsm, viscosity
Annotation:

Presented by us technology gives opportunity to prepare nanosized liposomes, where it may be incorporated as hydrophobic also hydrophilic molecules for suffer their biological activity when enter living organism. Such packing of molecules into phospholipid liposomes lead to increase possibilities to overcome the damaged organ cell membrane and penetrate into the cell cytoplasm. Therefore such nanoparticles are more effective for treatment diseases. Particularly, by this method we prepared liposomes using DPPC and DPPA lipids, inside of which were incorporated cholesterone, calcium ions and 24nm diameter gold nanoparticles. The common approach were used for preparing all such complex liposomes. Particularly, take into account whether the ligand dissolves in water or in organic solvent on the first stage it is necessary the interaction of ligands with lipids, afterwards it should be added the water (buffer) with temperature slightly above of phase transition temperature of liposomes. Then during one minute the mixture should be shaked intensively. In such way we have got the suspension of complex liposomes. At the last stage using the extruder we get complex liposomes with wishful diameters. As proof that we have got the complex liposomes with presented method the Zetasizer, calorimetric and spectrophotometric method were applied. At the end we want to mention that the presented method is different from those which were used before for preparing complex vesicles. This technology is easy, fast and economic, therefore is cheap. The parameters of complex liposomes prepared by our methods are analogous with those liposomes prepared by previous methods. It needs half an hour to prepare complex liposomes using our method. The Zimm-Ckrozers modified viscosimeter will be used to study the formation of biofilms under the action of different biological active agents. This method allows to continually observe biofilm formation on the surface of liquid media. By this method we will compare the effects of antibiotics and bacteriophages on biofilm formation. On a figure it is given the scheme of observation on biofilm formation dynamic, where the area of biofilm is indicated with arrows. Rotor is hunging into bacterial suspension by surface tension force (in the upper part of the figure), if bacteria starts biofilm formation the speed of rotation of the rotor will reduce.