Separation of enantiomers of chiral compounds with novel core-shell type polysaccaride-based chiral stationary phase in ultra high performance liquid chromatography using methanol mobile phase

Author: Lia Bezhitashvili
Co-authors: Ana Bardavelidze, Teona Orjonikidze
Keywords: superficially porous particles, Methanol, trans-Stilbene Oxide, Van-Deemter Curve, Core-Shell
Annotation:

Chromatographic adsorbents based on superficially porous (also referred to as fused-core or core-shell) silica are rapidly establishing themselves as the most promising media for achiral separations in high-performance liquid chromatography (HPLC). Some of their advantages are faster mass transfer between the mobile and stationary phase and more uniform particle size distribution leading to more homogeneous packed beds (resulting in higher plate numbers), as well as enabling faster separations without significant loss in column performance. Although a few studies have been published on the use of core-shell silica particles for enantioseparations in HPLC, their potential as supports for the preparation of chiral stationary phases (CSP) has not been adequately studied yet [1-3]. The use of core-shell silica-based stationary phases has been successfully demonstrated in various separation modes (RP, HILIC, etc.) and for both small and large molecules. While fast mass-transfer is expected to have a more significant impact in the analysis of large molecules (e.g. proteins), in practice core-shell silica is used extensively for the analysis of small molecules (such as pharmaceuticals, environmental pollutants, etc.). This success in the analysis of low relative molecular mass species has been attributed primarily to a significant decrease in the value equivalent to a theoretical plate (HETP) brought about by limited eddy dispersion and molecular diffusion contributions for columns made with core-shell particles. In this study the separation performance of chiral stationary phases (CSPs) made of polysaccharide-based chiral selectors coated onto superficially porous (core-shell or fused-core) silica supports were evaluated. High column performance reaching 200 000 plates per meter was observed

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